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Image Search Results
Journal: PLoS ONE
Article Title: Sensitivity of Human Intrahepatic Cholangiocarcinoma Subtypes to Chemotherapeutics and Molecular Targeted Agents: A Study on Primary Cell Cultures
doi: 10.1371/journal.pone.0142124
Figure Lengend Snippet: Sequences of primer pairs (sense and antisense, respectively) used for amplifying the genes of interest (GOI) and the internal reference gene (GAPDH) used for their nor Primer designed by the PROBEFINDER software ( https://www.roche-applied-science.com/sis/rtpcr/upl/index.jsp ).
Article Snippet: Cells were rinsed twice with PBS buffer for 2 min, blocked and incubated 1 hour with the following antibodies: Vimentin mouse monoclonal IgG1 (sc-32322, Santa Cruz Biotechnology), α-SMA mouse monoclonal IgG1(M0851, Dako), E-Cadherin mouse monoclonal IgG1 (sc-21791, Santa Cruz Biotechnology), CD326/EpCAM mouse monoclonal IgG1 (sc-59782, Santa Cruz Biotechnology), CD133/PROM1 mouse monoclonal IgG1 (TA309943, Origene),
Techniques: Software, Amplification
Journal: PLoS ONE
Article Title: Sensitivity of Human Intrahepatic Cholangiocarcinoma Subtypes to Chemotherapeutics and Molecular Targeted Agents: A Study on Primary Cell Cultures
doi: 10.1371/journal.pone.0142124
Figure Lengend Snippet: (A) Immunohistochemical and immunofluorescence analyses of mixed- and mucin-IHCCA primary cultures for Vimentin, α-SMA, E-Cadherin and the “epithelial” cancer stem cell markers CD133+, EpCAM+, LGR5+ and for IL6. A diffuse positivity for mesenchymal markers (Vimentin, α-SMA) and IL6 was observed while E-Cadherin was virtually negative and less than 5% cells where positive for the “epithelial” cancer stem cell markers. Representative experiment of N = 12 independent staining performed in separate primary cultures. (B) Flow cytometry analyses of primary cultures of mixed- and mucin-IHCCA (20–30 passages), labeled with anti-CD13, anti-CD90, anti-EpCAM, anti-CD133, and anti-LGR5 antibodies. Bar graphs and representative plots. Cells positive for CD13 and CD90 largely predominated with respect to CD133, EpCAM and LGR5. CD13+ cells predominated in mixed-IHCCA with respect to mucin-IHCCA, while the opposite was found for CD90+ cells. Mean ± SD of N = 18 independent experiments. * = p< 0.05 vs mucin-IHCCA; & = p< 0.01 vs mixed-IHCCA.
Article Snippet: Cells were rinsed twice with PBS buffer for 2 min, blocked and incubated 1 hour with the following antibodies: Vimentin mouse monoclonal IgG1 (sc-32322, Santa Cruz Biotechnology), α-SMA mouse monoclonal IgG1(M0851, Dako), E-Cadherin mouse monoclonal IgG1 (sc-21791, Santa Cruz Biotechnology), CD326/EpCAM mouse monoclonal IgG1 (sc-59782, Santa Cruz Biotechnology), CD133/PROM1 mouse monoclonal IgG1 (TA309943, Origene),
Techniques: Immunohistochemical staining, Immunofluorescence, Staining, Flow Cytometry, Labeling
Journal: PLoS ONE
Article Title: Sensitivity of Human Intrahepatic Cholangiocarcinoma Subtypes to Chemotherapeutics and Molecular Targeted Agents: A Study on Primary Cell Cultures
doi: 10.1371/journal.pone.0142124
Figure Lengend Snippet: RT-PCR analysis of Vimentin and cancer stem cell surface markers.
Article Snippet: Cells were rinsed twice with PBS buffer for 2 min, blocked and incubated 1 hour with the following antibodies: Vimentin mouse monoclonal IgG1 (sc-32322, Santa Cruz Biotechnology), α-SMA mouse monoclonal IgG1(M0851, Dako), E-Cadherin mouse monoclonal IgG1 (sc-21791, Santa Cruz Biotechnology), CD326/EpCAM mouse monoclonal IgG1 (sc-59782, Santa Cruz Biotechnology), CD133/PROM1 mouse monoclonal IgG1 (TA309943, Origene),
Techniques:
Journal: Scientific Reports
Article Title: Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX
doi: 10.1038/srep10326
Figure Lengend Snippet: ( a ) CR-CSCs form suspension cultured tumorspheres from culture day one to day five; ( b ) anti-CD44 fluorescence immunostaining analysis with CR-CSCs after enrichment suspension culture and colorectal cancer cells (HCT-8); ( c ) anti-CD133 fluorescence immunostaining analysis with CR-CSCs after enrichment suspension culture and HCT-8.
Article Snippet: USA.) was applied for CD44 staining and a
Techniques: Suspension, Cell Culture, Fluorescence, Immunostaining
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: List of Antibodies Used for IHC and IF
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques:
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Sequences of Primer Pairs (Sense and Antisense, Respectively) Used for Amplifying the Genes of Interest and the Internal Reference Gene ( GAPDH ) Used for Their Normalization
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: Amplification
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Flow cytometry (FC) analyses of established cell lines of human cholangiocarcinomas (CCAs). A: Bar graphs showing the distribution, as percentages, of cancer stem cell (CSC) subpopulation in established human cancer cell lines as assessed by FC for CSC markers. B: FC representative plots. We investigated: i) HuH-28 and CCLP-I (mucin-negative) and HUCCT-1 (mucin-positive) cell lines derived from human IHCCA; ii) TFK-1 (mucin-positive) cell line derived from pCCA; and iii) Mz-ChA-1 (mucin-positive) cell line derived from gallbladder carcinoma. CD44 expression is high in most of the cell lines (>74%). CD90+ cells are evident primarily in HuH-28 and CCLP-1. EpCAM positivity characterizes the predominant cell population in the intrahepatic (IHCCA) cell line, HUCCT-1, in perihilar (pCCA) cell lines, TFK-1 and, in gallbladder cancer cell line, Mz-ChA-1. CD133 and LGR5 are minimally expressed. C: Bar graphs showing the distribution, as percentage, of CSC subpopulations coexpressing CSC markers. D: FC representative plots. CD44+/CD90+ cells predominate in HuH-28 and CCLP-1 cell lines. CD44+/EpCAM+ cells are prevalent in HUCCT-1, TFK-1, and Mz-ChA-1 cell lines. CD13+/CD90+ cells predominate only in HuH-28 cells. ∗∗P < 0.01 versus the other cell lines; ††P < 0.01 versus HuH28.
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: Flow Cytometry, Derivative Assay, Expressing
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Immunophenotypic traits of human mucin-intrahepatic (IHCCA) and mixed-IHCCA. A: Periodic-acid Schiff (PAS) stain and immunohistochemistry (IHC) for keratin (K)7, EpCAM, LGR5, CD133, CD13, CD90, and α-smooth muscle actin (α-SMA) on human mucin-IHCCAs. Mucin-IHCCAs are diffusely positive for PAS, K7, EpCAM, LGR5, and CD133. By contrast, CD13 is restricted to a small subpopulation of tumor epithelial cells (arrow). CD90 and α-SMA are primarily expressed by tumor stromal cells (arrows). B: PAS stain and IHC for K7, EpCAM, LGR5, CD133, CD13, CD90, and α-SMA on human mixed-IHCCAs. Mixed-IHCCAs are diffusely positive for K7, EpCAM, CD13, and CD133. By contrast, LGR5 is restricted to a small subpopulation of tumor epithelial cells (arrows). CD90 and α-SMA were primarily expressed by tumor stromal cells (arrows). C: Double immunofluorescence (IF) for Desmin (red) and CD133 (green) in mucin-IHCCA; double IF for α-SMA (red) and pan-cytokeratin (Pan-CK; green) in mixed-IHCCAs. Approximately 10% of tumor epithelial cells (CD133+ or Pan-CK+, in green) express mesenchymal markers (desmin or α-SMA, in red) both in mucin- and in mixed-IHCCA. Yellow arrows indicate cells coexpressing epithelial and mesenchymal markers (yellow cells in merged images). Nuclei are shown in blue. Separate channels are shown. Boxed areas are shown magnified in the insets (bottom panels). D: Upper panels: IHC for SNAIL and Twist in human CCA specimens shows the expression of the two antigens within the nuclei of epithelial CCA cells (arrows). Lower panels: Double IF for SNAIL (red) and Pan-CK (green) in human CCA specimens confirms the expression of SNAIL within nuclei of epithelial CCA cells (arrows). Double IF for SNAIL (red) and α-SMA (green) in human CCA specimens shows the presence of CCA cells expressing both α-SMA and SNAIL. Images from mixed-IHCCA samples are displayed. E–J: IHC for CD133, CD13, and CD90 on human nontumor tissue surrounding CCA specimens. CD133 expression (E and F) is restricted to a few cells within Canals of Hering (CoH) and bile ductules (E, arrow) and to peribiliary glands (PBGs) within larger bile ducts (F, arrows). CD90 (G and H) is mostly expressed by stromal cells in the portal spaces (G, arrow) and around PBGs (H, arrows). CD13 (I and J) is diffusely expressed by hepatocytes, cells within CoH/bile ductules (I, yellow arrows), cholangiocytes of interlobular bile ducts (I, red arrow), and PBGs (J, arrow). Original magnification: ×20 (A–C, D, lower panels); ×40 (D, upper panel).
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: Staining, Immunohistochemistry, Immunofluorescence, Expressing
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Human cholangiocarcinoma (CCA) primary cultures. A: Immunohistochemical analyses of CCA primary cultures after 2 to 3 passages (P2/P3) and 20 to 30 passages (P > 20). The expression of mesenchymal and epithelial-mesenchymal transition markers (vimentin, α-SMA, SNAIL, Twist, S100A4, P-cadherin) largely predominate over that of epithelial markers (CD133, EpCAM, LGR5, E-cadherin) in all passages examined. During progression of primary cultures (P > 20 versus P2/P3) SNAIL- and Twist-positive cells tend to increase, whereas EpCAM-, LGR5-, and CD133-positive cells tend to decrease. Similar diffuse positivity for vimentin, α-SMA, and S100A4 is observed in all passages. B–D: Flow cytometric (FC) analyses of primary cultures (20 to 30 passages) obtained from mixed-IHCCA (IH-mixed), mucin-IHCCA (IH-mucin), and mucin-pCCA (p-mucin) subtypes; representative FC plots. B: Cells positive for CD13, CD44, and CD90 largely predominate with respect to CD133, EpCAM, and LGR5. CD13+ and CD44+ cells predominate in mixed-IHCCA with respect to mucin-IHCCA or mucin-pCCA, whereas the opposite is found for CD90+ cells. C: Cells double-positive for CD13 and CD44 (CD13+/CD44+) predominate in mixed-IHCCA with respect to mucin-IHCCA or mucin-pCCA. D: CD90+/CD44−/CD13− cells predominate in mucin-IHCCAs and mucin-pCCA with respect to mixed-IHCCAs. In these experiments, CD90+ cells are gated and further analyzed for the expression of CD44 and CD13 markers. ∗P < 0.05, ∗∗P < 0.01 versus mucin-CCAs. Original magnification: ×40 (A).
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: Immunohistochemical staining, Expressing
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Spheroids formed by cholangiocarcinomas (CCAs) subpopulations. A: We determined the capacity of spheroid formation by cancer stem cell (CSC) subpopulations immunosorted from CCA primary cultures; the number of spheroids (see bar graph) formed in vitro being a self-renewal index. By immunofluorescence (IF) for the marker used for immunosorting, we tested the purity of the formed spheroids. Spheroids formed by CD90+ cells are enriched for CD90+ cells, whereas the negative counterpart CD90− forms spheroids where CD90+ cells are <5%, indicating that these spheroids are formed by other CSC subpopulations. Each subpopulation forms spheroids efficiently, reaching a size of 100 to 500 μm after 7 days in culture. IF (nuclei were stained with DAPI) shows the enrichment in the spheroids by the immunosorted cell subpopulation. CD133, EpCAM, and LGR5 form a higher (P < 0.01) number of spheroids compared to cells expressing the mesenchymal cell marker, CD90, or CD13 (note that scales are different). CD13+ cells from mixed-IHCCA (IH-mixed) form a higher number of spheroids than CD13+ cells immunoselected from mucin-IHCCA (IH-mucin), whereas the opposite is observed for CD90+ or CD133+ cells (mucin > mixed.) B: Spheroids formed by CD133+ and CD90+ CSC subpopulations were analyzed by IF for markers of epithelial-mesenchymal transition (Twist, SNAIL, vimentin, P-cadherin). Spheroids show positive staining for Twist, SNAIL, vimentin, and P-cadherin without differences between CD133+ and CD90+ spheroids. ∗P < 0.05, ∗∗P < 0.01. Original magnification: ×30 (A); ×40 (B).
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: In Vitro, Immunofluorescence, Marker, Staining, Expressing
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Tumor Xenografts Obtained 2 Months after S.C. Injection of Spheroids Prepared from Different Subpopulations of Immunosorted CSCs
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: Injection
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Histology of s.c. human tumor xenografts. A: Hematoxylin and eosin (H&E): Xenografts arising from CD90+, EpCAM+, and LGR5+ spheroids are characterized by the presence of larger necrotic areas in comparison with tumors obtained from CD133+ cells, whereas in xenografts from CD13+ spheroids, only few and restricted necrotic areas are observed. Necrotic areas are encircled by the dotted line. B: H&E: All s.c. xenografts comprise of nests of pleomorphic cells with giant and irregular nuclei and multiple prominent nucleoli. Numerous tumor cells are PCNA positive. Most tumor cells are α-SMA positive (arrows), whereas few and scattered nests of tumor cells express K19 (arrows). C: Spheroids from mucin-IHCCA, injected s.c., give rise to xenografts in which neoplastic cells are surrounded by a Periodic-acid Schiff (PAS)-positive material (arrows). D: EpCAM+ and LGR5+ spheroids from mucin-IHCCA show the presence of few areas composed of K19+ duct-like structures (arrows). E: Spheroids from mixed-IHCCA give rise to xenografts that are almost PAS negative. F: 5% to 30% of cells are K19 positive and form few ductular-like structures (arrows). Original magnification: ×10 (A); ×40 (B–F).
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: Injection
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Summary of Morphological and IHC Features of S.C. Xenografts ∗
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques:
Journal: The American Journal of Pathology
Article Title: Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas
doi: 10.1016/j.ajpath.2015.02.010
Figure Lengend Snippet: Intrahepatic tumor xenografts: morphological and phenotypic features. Injection of spheroids (approximately 10,000 cells) formed by cells immunoselected for a determined cholangiocarcinoma (CCA) marker into the livers of cirrhotic SCID mice (carbon tetrachloride–induced) leads, after 4 weeks, to evident liver cancers. A: Tumor masses observed 4 weeks after intrahepatic injection of CD90+ spheroids from primary cultures of mucin-IHCCA. B: Hematoxylin and eosin (H&E): The liver is occupied by several tumor masses (dotted line); vascular invasion (arrows). C: H&E: Tumor masses were composed of strands of polygonal cells with giant nuclei and prominent nucleoli. D: Immunohistochemistry (IHC): At the center of tumor masses, α-SMA–positive tumor cells are present (arrows). E–H: At the periphery of the tumor masses, Periodic-acid Schiff (PAS)-positive cells (F, arrows), cords of HepPar-1–positive cells (G, arrows), and K19 ductular-like structures (H, arrows) are present, reproducing a moderately differentiated carcinoma. E = PAS staining: Area in the box is magnified in F; F = PAS staining: G = IHC for HepPar-1. H = IHC for CK19. I and J: Immunocompetent cirrhotic BALBc mice, under pharmacological immunosuppression, injected with CD133+ spheroids prepared from mucin-IHCCA primary cultures. Four weeks after intrahepatic injection, a tumor almost totally composed of PAS+ duct-like structures (arrows) is observed. I = H&E: J = PAS staining. n = 4 (I and J, mucin-IHCCA primary cultures). Original magnification: ×10 (B and E); ×20 (C, D, H, and I); ×40 (F, G, and J).
Article Snippet: For all immunoreactions, negative controls (the primary antibody was replaced with preimmune serum) were also included. shows the details of antibodies used in the study. table ft1 table-wrap mode="anchored" t5 caption a7 Name Host/isotype Source Catalog# Dilution CD326/EpCAM Mouse IgG1 Santa Cruz Biotechnology (Dallas, TX) sc-59782 1:50 K7 (cytokeratin 7) Mouse IgG1 Dako M7018 1:100 K19 (cytokeratin 19) Mouse IgG1 Abcam (Cambridge, UK) ab87014 1:50 K19 (cytokeratin 19) Mouse IgG1 Dako M0888 1:100 HepPar-1 Mouse IgG1 Dako M7158 1:50 CD133/Prominin 1 Rabbit IgG Abnova (Taipei, Taiwan) {"type":"entrez-protein","attrs":{"text":"PAB12663","term_id":"1236625334","term_text":"PAB12663"}} PAB12663 1:100
Techniques: Injection, Marker, Immunohistochemistry, Staining